Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice
Yamei Ma, Jianyuan Yang , Jingfang Dong, Shaohong Zhang, Wu Yang, Junliang Zhao ,Tifeng Yang, Luo Chen, Lian Zhou, Jian Wang, Jiansong Chen , Wenhui Li, WeiWu,Qing Liu* and Bin Liu*
International Journal of Molecular Sciences
Abstract
Although it is known that
rice 14-3-3 family genes are involved in various defense responses, the
functions of OsGF14f in response to diseases have not been reported.
Here, we showed that the transcription of OsGF14f was significantly
induced by leaf blast infection, and the overexpression
of OsGF14f quantitatively
enhanced resistance to leaf blast and bacterial blight in rice. Further
analysis showed that the expression levels of salicylic acid (SA)
pathway-associated genes (PAL1, NH1, PR1a and PR10) in the
OsGF14f-overexpressing plants, were higher than those in wild-type
plants after inoculation with the blast isolate (Magnaporthe oryzae
Barr). In addition, the expression level of OsGF14f was significantly
induced after SA treatment, and higher endogenous SA levels were
observed in the OsGF14f -overexpressing plants compared with that in
wild-type plants, especially after blast challenge. Taken together,
these results suggest that OsGF14f positively regulates leaf blast and
bacterial blight resistance in rice via the SA-dependent signaling
pathway.
Keywords: GF14f; leaf blast resistance; bacterial blight resistance; SA pathway
