QTLs identification for nitrogen and phosphorus uptake-related traits using ultra-high density SNP linkage
Youqiang
Fu, Xuhua Zhong, Junfeng Pan, Kaiming Liang, Yanzhuo Liu, Bilin Peng, Xiangyu
Hu, Nongrong Huang*
Plant Science
Abstract
To
understand the genetic basis of nitrogen and phosphorus uptake in the
cultivated rice, quantitative trait loci (QTL) analysis for 7 nitrogen and
phosphorus uptake-related traits including above-ground biomass (AGB), leaf colour
value (SPAD) in heading stage, grain nitrogen concentration (GNC), grain
nitrogen content of the plant, total nitrogen content (TNC), grain phosphorus
concentration, total phosphorus content (TPC) were conducted using SNP markers
in a F2 population derived from a cross between GH128 and W6827. A total of 21
QTLs for nitrogen and phosphorus uptake-related traits distributed in 16
regions along 6 chromosomes were detected using a high density genetic map
consisting of 1582 bin markers, with QTLs maximum explaining 8.19% of the phenotypic
variation. Nine QTLs (42.9% of total QTLs) were detected on chromosome 2. Among
them, two QTL clusters including AGB, TNC, TPC and GNC were also detected in
the region bin 140 and bin 146 on the chromosome 2. The distance between the
two clusters was only 4.1 cM. The presence of QTL clusters has im[1]portant
significance and could be useful in molecular marker assisted breeding. These
genomic regions might be deployed for the simultaneous improving the use
efficiency of nitrogen and phosphorus in rice breeding.
Keywords: Quantitative
trait locus (QTL) F2 populations QTL mapping
Nitrogen Phosphorus Rice
